Parvalbumin: as a fish-allergen
نویسندگان
چکیده
منابع مشابه
Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy.
IgE-mediated reactions to fish allergens represent one of the most frequent causes of food allergy. We have constructed an expression cDNA library from carp (Cyprinus carpio) muscle in phage lambda gt11 and used serum IgE from a fish allergic patient to isolate 33 cDNA clones that coded for two parvalbumin isoforms (Cyp c 1.01 and Cyp c 1.02) with comparable IgE binding capacities. Both isoform...
متن کاملExposure to parvalbumin allergen and aerosols among herring processing workers.
BACKGROUND There are increasing reports of allergies and respiratory symptoms among workers in the fish processing industry, coinciding with an increasing use of high-pressure water in the processing plants. However, few studies have measured exposure in these work environments. OBJECTIVES The aim of this study was to characterize the occupational exposure of workers to herring antigen and to...
متن کاملRapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry.
Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (...
متن کاملFish β-parvalbumin acquires allergenic properties by amyloid assembly.
PRINCIPLES Amyloids are highly cross-β-sheet-rich aggregated states that confer protease resistance, membrane activity and multivalence properties to proteins, all essential features for the undesired preservation of food proteins transiting the gastrointestinal tract and causing type I allergy. METHODS Amyloid propensity of β-parvalbumin, the major fish allergen, was theoretically analysed a...
متن کاملThe use of recombinant fish parvalbumin Gad c 1 in the characterisation of fish allergic patients
Material and methods We selected 55 pts (34M, 21F; average age:7.4 years). All had fish allergy, characterized by positive clinical history, skin-prick tests and serum specific IgE to several fish and Gad c 1 (UniCap, Thermo-Fisher). Oral food challenge was performed to evaluate fish tolerance acquisition. Statistical analysis was performed using GraphPad Prisma version 5.0 (GraphPad Software I...
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ژورنال
عنوان ژورنال: Frontiers in Immunology
سال: 2013
ISSN: 1664-3224
DOI: 10.3389/conf.fimmu.2013.02.00526